Sturgeons in large rivers: detecting the near-extinct needles in a haystack via eDNA metabarcoding from water samples

نویسندگان

چکیده

Abstract Sturgeon populations are declining worldwide and the target of extensive conservation efforts. Addressed in several pieces legislation, sturgeons have received considerable attention as flagship or umbrella species. Despite need for a better understanding distribution population status, use traditional sampling methods failed past, thereby hampering reliable assessments, prerequisite conservation. Here, we describe development application an environmental DNA (eDNA) metabarcoding approach detecting rare large rivers. Exemplarily, developed reference database five native Danube ( Acipenser stellatus , gueldenstaedtii ruthenus, nudiventris, Huso huso ) two non-native species baerii transmontanus ), assessed these ex situ, used eDNA detection tool along entire length (Europe, ~ 2850 km) major tributaries. In situ analyses, all assays yielded positive amplifications sturgeon Danube, presence A. ruthenus was confirmed at 14 29 sites (48.3%), 2 18 tributary (11.1%), providing first comprehensive large-scale biogeographical snapshot this Relative number reads assigned to varied between 0 2.5%, with registering detections being clustered 3 sections Danube. Our findings enabled us confirm advantages monitoring over whole-river studies conducted on geographical scale, therefore consider it be promising measures, fisheries management, scientific studies, adaptive management plans global scale.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Needles in a haystack

supplements, as in the case of vita­ min a. yet, other studies suggest that the main impact of poverty extends beyond diet and cannot be addressed through improved nutrition alone. Such is the expe­ rience from peru, where a study by the tuberculosis research group at imperial college (london, uK) has been evaluat­ ing whether dietary supplementation with vitamin a, vitamin D and zinc reduces t...

متن کامل

The Needles-in-Haystack Problem

We consider a new data mining problem of detecting the members of a rare class of data, the needles, that have been hidden in a set of records, the haystack. Besides the haystack, a single instance of a needle is given. It is assumed that members of the needle class are similar according to an unknown needle characterization. The goal is to find the needle records hidden in the haystack. This p...

متن کامل

Proteolytic needles in the cellular haystack.

2. Hille, B. Ion Channels of Excitable Membranes 3rd edn., 131–160 (Sinauer, Sunderland, Massachusetts, 2001). 3. Shi, G. et al. Neuron 16, 843–852 (1996). 4. Accili, E.A. et al. J. Physiol. (Lond.) 512, 325–336 (1998). 5. Gu, C. et al. Science 301, 646–649 (2003). 6. Rettig, J. et al. Nature 369, 289–294 (1994). 7. Gulbis, J.M. et al. Cell 97, 943–952 (1999). 8. Weng, J. et al. J. Biol. Chem. ...

متن کامل

Atypical chest pain: Needles in a haystack.

A 20-year-old man presented with a 6-month history of intermittent chest pain. Initial imaging demonstrated approximately 15 sewing needles lodged in his myocardium, predominantly in the left ventricle. The patient has been referred to cardiothoracic surgery for further management. His progress will be monitored closely.

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Biodiversity and Conservation

سال: 2022

ISSN: ['1572-9710', '0960-3115']

DOI: https://doi.org/10.1007/s10531-022-02459-w